A simple and precise visible spectrophotometric method was developed for the determination of lidocaine hydrochloride in bulk and pharmaceutical preparations based on the formation of green colored molecular complex with sodium nitroprusside in presence of hydroxyl amine hydrochloride under alkaline conditions and exhibiting λmax at 401 nm. The Regression analysis of Beer’s Law plot showed good correlation in a general concentration range of 10-50µg/ml with correlation coefficient (r = 0.998). The proposed method is validated with respect to accuracy, precision, and linearity, limit of quantification and limit of detection. The suggested procedure is successfully applied to the determination of the drug in pharmaceutical preparation, with high percentage recovery of 100%, 98.19%, 99.12%. The results of analysis have been validated according to ICH guidelines. The results are found satisfactory and reproducible. The method is applied successfully for the estimation lidocaine hydrochloride in tablet form without the interference of excipients.
Loading....